Coding

Part:BBa_I728951:Design

Designed by: aditya kohli   Group: iGEM07_MIT   (2008-04-30)


Human Matrix Metalloproteinase 1 (Mmp1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 53
    Illegal SpeI site found at 724
    Illegal PstI site found at 738
    Illegal NotI site found at 7
    Illegal NotI site found at 731
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 533
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 724
    Illegal PstI site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to change nucleotide 710 from a C to a T in order to get rid of a PstI site. I also added an ATG codon to the beginning of the sequence because this sequence is the active, cleaved form of Mmp1 and it doesn't have a start codon in it.


Source

From human origin

References